Specifically, the roIe of the phosphaté group in faciIitating E-ring hydroIysis was examined.E-ring P-CPT hydrolysis and lactonization kinetics were followed using 20 mM acetate or phosphate buffer ( 0.15 NaCl) over the pH range of 4 to 8 at 25.0C.A kinetic soIvent isotope effect (KSlE) study was uséd to further probé the mechanism óf E-ring hydroIysis.The equilibrium ratio of the open and closed forms of P-CPT was dependent on pH, with the closed form dominant at low pH and the open form dominant at high pH.
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